Affinity Chromatography: Methods and Protocols (Methods in Molecular Biology)

Details powerful affinity chromatography methods, ranging from traditional affinity purification such as immunoaffinity chromatography, to the use of the latest phage-display technology in the discovery of affinity ligands and drugs. Also included are separations of small molecules such as haptens, protein ligands, and supramolecular structures. Each chapter is devoted to a specific technique and includes an introduction, an explanation of principles, a detailed materials list, and instructions. Practical notes suggest alternative procedures and describe how to overcome problems.