Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis. Because the protein standards and “unknowns” are prestained, the separation of proteins can be observed during electrophoresis. Included in the experiment is EDVOTEK’s formulation of protein grade agarose, which provides an alternative to the use of polyacrylamide gels. Kit includes: instructions, prestained LyphoProtein samples, gel loading solution, agarose, electrophoresis buffer, stain, SDS solution. You need: horizontal electrophoresis apparatus, power supply, white light visualization system, automatic micropipet with tips, microwave oven or hot plate, waterbath, metric rulers, lab glassware, methanol, glacial acetic acid, distilled or deionized water.
Features
- For 12 gels; gel prep 40 min, electrophoresis 60 min
- Kit includes: instructions, prestained LyphoProtein samples, gel loading solution, agarose, electrophoresis buffer, stain, SDS solution
- You need: horizontal electrophoresis apparatus, power supply, white light visualization system, automatic micropipet with tips, microwave oven or hot plate
- All you need: waterbath, metric rulers, lab glassware, methanol, glacial acetic acid, distilled or deionized water
- Introduce a simple method to determine protein subunit molecular weights using horizontal electrophoresis
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