This experiment introduces the use of restriction enzymes as a tool to digest DNA at specific nucleotide sequences. Bateriophage lambda DNA has a linear structure and 6 Eco RI recognition sites. Separation by agarose gel electrophoresis of an Eco RI digest of lambda DNA will yield 6 bands (5 distinct bands, two are very close in size) corresponding to the DNA fragments.
Features
- Kit Includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain, calibrated pipet, 100 ml graduated cylinder, and microtipped transfer pipets.
- All You Need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, 65@C waterbath, visualization system.
- For 8 Gels.
- Complete in 60 min.
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