Flash-Extract PCR Kit provides for an ultra-fast, low-cost and highly efficient method of DNA extraction and amplification from a variety of solid tissues, including mouse tails and ear punches, plant leaves, bacteria and saliva. Flash-Extract PCR Kit includes a proprietary single-tube extraction solution which releases genomic DNA in only 8 minutes, followed by PCR amplification using Azura ExtremeTaq HiFi Red Mix for high performance end-point PCR. ExtremeTaq HiFi Red Mix is a 2x hot-start mixture which also includes an inert red dye to facilitate direct gel-loading. For the flexibility to accommodate various work-flows, PCR-ready genomic DNA can be used immediately in end-point PCR, used in real-time PCR with SYBR Green or TaqMan Probe based chemistry, or stored at -20degC.
Features
- DNA extraction and PCR from mouse tail, mouse ear, plant leaves, bacteria and saliva.
- Simple, ultra-fast tissue lysis followed by robust amplification which outperforms conventional methods and other commercial kits.
- Automation-friendly solution for extraction of PCR-ready genomic DNA in 8 minutes without laborious centrifugation steps.
- 1 Hour Work-flow: DNA Extraction and PCR can be complete in less than 60 minutes for improved turnaround time
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