Flourescence Microscopy of Living Cells in Culture, Part B: Quantitaive Flourescence Microscopy-Imaging and Spectroscopy (Volume 30) (Methods in Cell Biology, Volume 30)

Only recently has the true power of fluorescence techniques evolved for use with single living cells. The present status of the field reflects the occurrence of a revolution in cell biological research. Volume 30, along with Volume 29, provide the cell biologist with a sourcebook of methods. Volume 29 deals with the preparation, delivery, and detection of fluorescent probes. Volume 30 explores a combination of the theoretical and technical issues related to the quantitation of fluorescence signals in the living cell with a light microscope. Key Features * Convenient bench-top format Comprehensive methods included for: * Imaging function, systems, and measurement * Fluorescent standards and indicators * Fluorescence ratio imaging, resonance energy transfer, and other microscopy methods * Design of instruments and interpretation of data * Applications to DNA, membranes, and other cellular components