Edvotek 109 DNA Fingerprinting I – I.D. of DNA by Restriction Fragmentation Patterns, for 8 Gels

Basic concepts of DNA fingerprinting are featured in this lab by comparing crime scene DNA with suspect DNAs. Fingerprint patterns are separated by agarose gel electrophoresis and the students determine who may have done-it. Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain, calibrated pipet, 100ml graduated cylinder, and microtipped transfer pipettes. All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system. DNA samples are stable at room temperature. However, if the experiment will not be conducted within one month of receipt, it is recommended that the DNA samples be stored in the refrigerator. DNA samples do not require heating prior to gel loading. Ready-to-Load DNA samples for electrophoresis: A: DNA from crime scene cut with Enzyme 1, B: DNA from crime scene cut with Enzyme 2, C: DNA from Suspect 1 cut with Enzyme 1, D: DNA from Suspect 1 cut with Enzyme 2, E: DNA from Suspect 2 cut with Enzyme 1, F: DNA from Suspect 2 cut with Enzyme 2.

Features

• For 8 gels; complete in 60 minutes
• Kit includes: instructions, Ready-to-Load DNA samples, UltraSpec-Agarose powder, practice gel loading solution, electrophoresis buffer, InstaStain Blue and FlashBlue stain
• Kit includes: calibrated pipet, 100 ml graduated cylinder, and microtipped transfer pipettes
• All you need: electrophoresis apparatus, power supply, automatic micropipet and tips, balance, microwave or hot plate, visualization system
• Basic concepts of DNA fingerprinting are featured in this lab by comparing crime scene DNA with suspect DNAs