This economical 2-part exercise provides an introduction to agarose gel electrophoresis. In the first part of the exercise, students identify unknown dye molecules by comparing their electrophoretic migration with the migration of known dyes. In the second part students identify dye molecules that bind to DNA and determine the mechanism. The exercise was designed for 8 groups of students and includes four colorful dye mixtures and DNA. Contents included: Complete Instructions Sample 1 Sample 2 Sample 3 Sample 4 DNA (8) 1ml Transfer Pipets Agarose (5 Grams) TG Buffer (Contents makes 3.5 Liters of re-usable buffer) Accessories Needed: Electrophoresis Equipment Automatic Pipeters (Optional) Set up for 16 Students working in pairs. Approximately(2) 40 – 60 minute lab periods.
Features
- Includes complete instructions, 4 samples and DNA
- (8) 1ml Transfer Pipets
- Agarose (5 Grams)
- TG Buffer (Contents makes 3.5 Liters of re-usable buffer)
- Requires Electrophoresis Equipment
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